Basic Violet 14
Histological and Biological Applications: Basic fuchsin demonstrates utility in biological research, botanical studies, and microbiological identification protocols, including nucleic acid verification procedures and Mycobacterium tuberculosis detection methodologies.
Tissue Staining Performance: The dye effectively coloration collagen structures, elastic fiber networks, and selected histological specimens, establishing its value in histological analysis and microbial characterization.
Solubility Characteristics: Basic fuchsin exhibits dissolution capacity in both aqueous and ethanolic media, enabling flexible preparation of staining solutions for various laboratory requirements.
Storage Stability: The compound maintains staining efficacy when preserved under light-restricted conditions with appropriate pH regulation, ensuring consistent performance in diagnostic and research applications.
This category comprises aniline-based colorants, also known as coal-tar derivatives, which encompass numerous variants with broad application scope. A principal limitation involves their photodegradation susceptibility, particularly evident in shades such as aniline blue and triarylmethane derivatives including brilliant green and methyl green. Solution preparation requires meticulous pH control and protection from ultraviolet radiation; under appropriate storage conditions, these colorants can maintain chromatic stability for multiple years without substantial deterioration.
Melting point | 250 °C |
Boiling point | 300℃[at 101 325 Pa] |
density | 0.999 g/mL at 20 °C |
bulk density | 500kg/m3 |
vapor pressure | 0Pa at 25℃ |
refractive index | n20/D 1.334 |
Fp | 48 °F |
storage temp. | 2-8°C |
solubility | H2O: soluble1mg/mL |
form | liquid |
Colour Index | 42510 |
color | Green to dark green |
PH | 5-6 (1g/l, H2O, 25°C) |
Water Solubility | 4 G/L (25 ºC) |
λmax | 543 nm |
Merck | 14,5652 |
BRN | 4166684 |
Biological Applications | Detecting cancer; treating Jock itch,oral fungal infection,piles; periodontal disease |
LogP | 1.632 at 25℃ |
IARC | 2B (Vol. Sup 7, 57, 99, 100F) 2012 |
EPA Substance Registry System | C.I. Basic Violet 14 (632-99-5) |
Safety Information
Hazard Codes | F,Xn |
Risk Statements | 40-22 |
Safety Statements | 7-16-36/37 |
WGK Germany | 3 |
RTECS | CX9850000 |
TSCA | Yes |
HS Code | 3204 13 00 |
Hazardous Substances Data | 632-99-5(Hazardous Substances Data) |
Basic fuchsin is an alkaline dye that presents as a dark red crystalline powder or particulate solid. It demonstrates aqueous solubility at 1% concentration and ethanol solubility at 8% concentration. This coloring agent finds extensive application in biological specimen preparation, where it effectively stains collagen fibers, elastic fibers, fuchsin granules, and neural nuclei in central nervous system tissues. In botanical microscopy, it serves to highlight lignified cell walls in vascular plants and functions as a comprehensive staining agent for protozoans and charophyte specimens.
In bacteriological diagnostics, basic fuchsin is routinely employed in the acid-fast staining procedure for Mycobacterium tuberculosis identification. This dye further functions as a critical Schiff-type reagent in the Feulgen histochemical technique for chromosomal DNA localization and verification. The chromogenic properties remain stable when preserved under light-protected conditions with maintained pH parameters.
